Abcam, ab24094, Anti-HLA B27 antibody [EP-4]

Size: 100µg
Mouse Monoclonal HLA B antibody. Suitable for ICC and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing HLA B27 protein.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:EP-4,
Isotype:IgM,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:ICCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab24094 recognizes the HLA B27 cell surface antigen on human cells. It may be used to HLA type human lymphoctyes. Approximately 60% of patients with ankylosing spondylitis are HLA B27 positive. This reagent can be used to help identify this HLA haplotype in human lymphocytes.

Properties and Storage Information:
Form-Liquid, Purity-IgM fraction, Storage buffer-pH: 7.3Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HLA-B27 also known as Human Leukocyte Antigen-B27 is a specific allele of the HLA-B gene. This protein is part of the major histocompatibility complex (MHC) class I molecules which play a role in the immune system by presenting peptide antigens to T cells. HLA-B27 has an approximate molecular weight of 44 kDa. It is expressed on the surface of most nucleated cells particularly on immune cells. The allele is well-studied for its association with certain inflammatory diseases.
Biological function summary
HLA-B27 presents peptides derived from intracellular proteins enabling cytotoxic T-lymphocytes to recognize and respond to pathogens or damaged cells. It forms a complex with beta-2 microglobulin and peptide antigens facilitating antigen presentation. The HLA-B27 antigen-presenting complex plays a role in immune surveillance by maintaining immune system recognition of foreign peptides and altered self-peptides.
Pathways
HLA-B27 is integral in the antigen processing and presentation pathway. This pathway involves the proteasome TAP transporters and other MHC class I molecules. The protein interacts with molecules like HLA-A and HLA-C within this pathway which also play similar roles in immune surveillance and response. These interactions ensure an effective immune response against intracellular pathogens.
HLA-B27 shows a strong association with ankylosing spondylitis and other spondyloarthropathies. Ankylosing spondylitis is a form of chronic inflammatory arthritis that affects the spine and large joints. In continued research HLA-B27-positive individuals demonstrate increased susceptibility to these disorders. The mechanisms might involve interactions with specific peptide antigens causing inappropriate immune responses. Related proteins such as anti-HLA-ABC FITC can assist in studying and identifying those cells presenting the HLA-B27 antigen aiding in diagnoses.