Abcam, ab24102, Anti-MRP1 antibody [MRPm5]

Size: 500µL
Mouse Monoclonal MRP1 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Human samples. Cited in 67 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ABCC1 aa 950-1250.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:MRPm5,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, Flow Cyt, IHC-Fr, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ABCC1 aa 950-1250. The exact immunogen used to generate this antibody is proprietary information.P33527,
Epitope:MRP1 antibody reacts with an internal epitope of MRP1.,
Specificity:MRPm5 reacts with an internal epitope of MRP1, a 180-195 kD transmembrane transporter protein overexpressed in varioushuman non-P-glycoprotein MDR tumor cell lines.MRPm5 does not cross-react with the human MDR1 and MDR3 gene products.

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: PBS, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MRP1 also known as ABCC1 is a transporter protein with a mass of approximately 190 kDa. This protein belongs to the ATP-binding cassette (ABC) transporter family. MRP1 actively exports a variety of substrates from cells by hydrolyzing ATP to ADP and inorganic phosphate. You can find MRP1 expressed in many tissues including the lung testis and kidney. It helps in cellular detoxification by exporting organic anions and other conjugated metabolites.
Biological function summary
This protein functions as an important determinant of multidrug resistance in cancer treatment. MRP1 can form part of a larger protein complex that includes other transporters like MRP2. Through its activity MRP1 protects tissues from toxic substances by transporting them out of the cell. Its role in transporting glutathione-conjugated compounds highlights its importance in cellular defense mechanisms against oxidative stress and xenobiotics.
Pathways
MRP1 participates in the glutathione metabolism and the xenobiotic efflux pathways. Both pathways involve cellular detoxification and the accumulation of anomalies can cause harmful effects in the body. MRP1 works with proteins such as GSTP1 which conjugates toxic substances with glutathione preparing them for export by MRP1. This coordination ensures efficient detoxification and protection of cells from damage.
MRP1 has associations with several conditions particularly drug resistance in various cancers and chronic obstructive pulmonary disease (COPD). In cancer MRP1 overexpression often results in reduced treatment efficacy due to chemotherapy drugs being expelled from the cell helping to resist their cytotoxic effects. Research indicates a connection between MRP1 and P-glycoprotein (ABCB1) in cancer drug resistance pointing to a broader resistive mechanism in which multiple transporters are involved.