Abcam, ab241080, Anti-RUFY1 antibody

Size: 100µg
Rabbit Polyclonal RUFY1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RUFY1 aa 650 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human RUFY1 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q96T51

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RUFY1 also known as RUN and FYVE domain-containing protein 1 is an intracellular protein that functions as a signaling adaptor playing a role in endosomal trafficking. The protein with a mass of approximately 79 kDa contains a RUN domain and a FYVE finger which are known for interactions with other molecules and membranes. RUFY1 is widely expressed in various tissues but shows higher levels in immune cells including macrophages and dendritic cells. Its localization in endosomes aids its role in vesicle dynamics and cellular traffic.
Biological function summary
The protein impacts immune response by regulating antigen presentation and inflammation. RUFY1 forms part of the molecular machinery responsible for endosomal transport interacting with other proteins to ensure the proper delivery of cargo within cells. This role is important in maintaining cellular homeostasis and facilitating the immune system's response to pathogens. By modulating endosomal pathways RUFY1 supports effective intracellular sorting and signaling which are important for immune cell function.
Pathways
RUFY1 actively contributes to the endocytic trafficking and NF-kB signaling pathways. In endocytosis it helps coordinate the transport and maturation of early endosomes collaborating with proteins like Rab5 and Rab7. In NF-kB signaling RUFY1 influences cellular inflammatory responses by affecting the activation and translocation of the NF-kB complex. These pathways emphasize its role in linking vesicle trafficking with key cellular processes that manage immune and inflammatory responses.
Alterations in RUFY1 function can affect immune system dynamics and contribute to inflammatory disorders and cancer development. Its association with rheumatoid arthritis highlights its involvement in chronic inflammatory states as RUFY1 impacts the NF-kB signaling pathway which is significant in this condition's pathology. In cancer dysregulation of RUFY1-related cellular transport and signaling can influence tumor progression with proteins like Rab GTPases also implicated in these disorders due to their mutual involvement in vesicle trafficking and signaling activities.