Abcam, ab241146, Human Growth Hormone Antibody Pair - BSA and Azide free

Size: 10 x 96Tests
Human Growth Hormone Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human Growth Hormone.
Key facts
Reacts with:Human,
Assay type:ELISA set,
Range:9.4 - 600 pg/mL,
Assay Platform:Reagents

Product details:
The Antibody Pair can be used to quantify Human Growth Hornmone. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (
ab190811
), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The pair can be used in variety of assays and platforms including but not limited to:
Sandwich ELISA
FRET/TR-FRET/HTRF
Meso Scale Discovery
Bead-based assays
AlphaLISA
/AlphaScreen
DELFIA
immunoassays
and Single Molecule Counting (SMC™) immunoassays
Multiplex
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
Concentration of ~1 mg/ml as measured by the protein A280 method.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody:
recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody:
recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration
: ~1 mg/ml
Storage buffer:
100% PBS
Liquid
Isotype:
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Growth Hormone (GH) also known as somatotropin is a polypeptide hormone with a mass of approximately 22 kDa. The pituitary gland synthesizes and secretes GH. Once synthesized GH circulates in the bloodstream and affects various tissues throughout the body. GH's expression is tightly regulated because of its significant roles in growth processes. Researchers might use anti-growth hormone antibodies and various hGH testing kits including ELISA to study its levels and functions.
Biological function summary
GH triggers growth and cell reproduction by exerting effects on multiple tissues. It binds to the growth hormone receptor which initiates a cascade of reactions that ultimately stimulates growth in muscle bone and cartilage. GH also contributes to protein synthesis and helps regulate metabolism. GH acts mostly independently but can interact with cytokines and other hormones.
Pathways
GH plays important roles in the regulation of the JAK-STAT signaling pathway and the IGF-1 signaling pathway. The JAK-STAT pathway is important for transmitting the signal of GH binding to its receptor into cellular responses. GH stimulates IGF-1 production in the liver and IGF-1 then acts in an endocrine manner to mediate much of GH’s growth-promoting activity. These interactions highlight the interconnectedness of GH with other hormones in growth signaling networks.
Several health conditions can arise from abnormal levels of GH. Dwarfism occurs due to GH deficiency while acromegaly results from GH excess. Clinical analysis might use hGH kits to measure hormone levels and aid in diagnosis. GH's role in such conditions links it to other hormones like insulin and glucagon that help regulate glucose levels further affecting metabolic processes. Recombinant forms of GH are used in therapy for these disorders providing avenues for treatment and management.