Abcam, ab241297, Anti-WDR4 antibody

Size: 100µL
Rabbit Polyclonal WDR4 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human WDR4 aa 350 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human WDR4 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P57081

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Antibody was affinity purified using an epitope specific to WDR4 immobilized on solid support., Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The WDR4 protein also known as WD repeat domain 4 functions in the modification of ribonucleic acids. It specifically takes part in the methylation process of N(7)-methylguanine-tRNA (m7G46) where it serves as an important component of the tRNA methyltransferase complex. With an approximate mass of 51 kDa WDR4 shows expression in various tissues throughout the human body contributing to fundamental cellular processes.
Biological function summary
WDR4 plays a role in the maintenance of accurate protein synthesis. It contributes to the stabilization and correct folding of tRNA molecules which are essential for the translation of genetic information from mRNA to proteins. As part of the tRNA methyltransferase complex WDR4 interacts with the METTL1 enzyme highlighting its involvement in precise tRNA modification processes critical for cellular function and proliferation.
Pathways
The function of WDR4 integrates into the broader landscape of the RNA modification pathway. This pathway is tied to the regulation of gene expression and cellular homeostasis. WDR4's activity impacts the efficiency and fidelity of the translation pathway. In this context WDR4 works closely with METTL1 enhancing the stability and functional capacity of tRNA during protein synthesis which is pivotal for the correct transmission of genetic code within cells.
WDR4's malfunction or altered expression associates with several conditions including microcephalic primordial dwarfism where its disruption affects brain development and growth. The protein interacts with METTL1 implicated in the same disorder demonstrating the importance of their coordinated function. Additionally alterations in WDR4 expression might link to certain types of cancers where aberrant tRNA methylation affects tumor growth and progression indicating the potential for WDR4 as a therapeutic target.