Abcam, ab241470, Anti-NuMA antibody

Size: 100µL
Rabbit Polyclonal NuMA antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NUMA1 aa 2050 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human NUMA1 aa 2050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q14980

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab241470 was affinity purified using an epitope specific to NuMA immobilized on solid support., Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NuMA also known as "nuclear mitotic apparatus protein" is a large protein with a mass of approximately 250 kDa. It localizes in the nucleus and becomes highly concentrated at the spindle poles during mitosis. NuMA proteins appear in a variety of cells especially in cells undergoing division. Researchers often refer to it by alternate names such as "anti NuMA" when discussing its interactions in cellular contexts. Scientists have developed products such as NuMA polyclonal antibodies to study this protein's function.
Biological function summary
The protein plays an important role in the organization of the mitotic spindle during cell division. It ensures that the spindle microtubules are correctly aligned and positioned. NuMA acts within a complex that includes dynein and dynactin facilitating proper chromosomal segregation. These actions are critical for maintaining genomic stability through accurate cell division.
Pathways
NuMA's involvement in the cell cycle is significant especially within the mitotic checkpoint control pathways. It interacts closely with proteins like pericentrin and LGN ensuring the efficient completion of mitosis. These interactions confirm the protein's importance in pathway regulation particularly during transition phases within the cell cycle.
Abnormal NuMA function has associations with cancer and certain genetic disorders like microcephaly. Changes in NuMA expression or malfunction can disrupt normal spindle formation leading to chromosomal instability a common hallmark of cancer cells. Its interaction with proteins like p53 further connects it to oncogenic processes suggesting that understanding NuMA's pathways could contribute to therapeutic strategies.