Abcam, ab241573, Anti-eIF2B4 antibody

Size: 100µL
Rabbit Polyclonal eIF2B4 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human EIF2B4 aa 50-100.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human EIF2B4 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.Q9UI10

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab241573 was affinity purified using an epitope specific to eIF2B delta/EIF2B4 immobilized on solid support., Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EIF2B4 also known as translation initiation factor eIF-2B subunit delta is an important component of the eIF2B complex. It plays a critical role in the regulation of protein synthesis by facilitating the exchange of GDP for GTP on eIF2. eIF2B4 has a molecular mass of approximately 67 kDa. This protein is ubiquitously expressed in various tissues indicating its importance in general cellular function.
Biological function summary
EIF2B4 functions within the eIF2B complex which is essential for the initiation of translation in eukaryotic cells. Its role in this complex involves the regulation of the eIF2 cycle which is important for controlling the rate of global protein synthesis. By mediating guanine nucleotide exchange on eIF2 eIF2B4 helps manage cellular responses to stress including the integrated stress response. This complex is vital in maintaining cellular homeostasis especially under conditions of amino acid deprivation or other stress factors.
Pathways
EIF2B4 plays a pivotal role in the integrated stress response (ISR) and is a part of the eukaryotic initiation factor 2 (eIF2) signaling pathway. These pathways adjust protein synthesis rates in response to varying environmental conditions and stress signals. eIF2B4 interacts with eIF2 and together they regulate translation by modulating the availability of eIF2-GTP. This interaction is essential for the precise control of protein synthesis during stress conditions which decides cell survival and adaptation.
EIF2B4 has a significant connection to Vanishing White Matter Disease (VWMD) and neurological disorders. Mutations in eIF2B4 can disrupt normal protein synthesis which leads to the onset of VWMD a progressive leukoencephalopathy. This pathology links eIF2B4 to disorders affecting the central nervous system. Additionally the protein relates to eIF2B5 within the context of these diseases since eIF2B5 mutations also contribute to similar pathologies suggesting their interaction is critical to neurological health.