Abcam, ab241949, Anti-HCP1/PCFT antibody

Size: 100µg
Rabbit Polyclonal HCP1/PCFT antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC46A1 aa 200-300.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human SLC46A1 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.Q96NT5

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Antibody was affinity purified using an epitope specific to HCP1/PCFT immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HCP1/PCFT also known as HCP1 HCP1 form or HCP-1 is a proton-coupled folate transporter. The protein has a mass of approximately 50 kDa and is expressed mainly in the duodenum and jejunum of the small intestine. It plays a central role in the absorption of dietary folate and folate metabolites. The presence of HCP1/PCFT is also noted in the liver and other tissues serving functions related to folate-dependent metabolic processes.
Biological function summary
HCP1/PCFT functions as a transporter that facilitates the absorption of folate across intestinal epithelial cells. It interacts with FOLR1 forming a functional complex contributing to folate uptake. This activity is essential for maintaining folate levels within cells influencing cell division and growth. The unique transport mechanism of HCP1/PCFT is pH-dependent operating optimally under acidic conditions found in the proximal intestine.
Pathways
HCP1/PCFT is involved in the folate and one-carbon metabolism pathways. It works closely with other transporters like RFC (Reduced Folate Carrier) and proteins such as DHFR (Dihydrofolate Reductase) to ensure efficient folate utilization. In the metabolic pathway these interactions are important for purine and pyrimidine synthesis which are necessary for DNA replication and repair.
Alterations in HCP1/PCFT function are linked to hereditary folate malabsorption and certain types of anemia. Impaired HCP1/PCFT activity can cause systemic folate deficiency leading to developmental issues and hematological abnormalities. The transporter is also connected to cancer as folate metabolism impacts tumor growth involving proteins like TYMS (Thymidylate Synthase) in its metabolic network.