Abcam, ab241957, Anti-Ajuba antibody

Size: 100µg
Knockout Tested Rabbit Polyclonal AJUBA antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human AJUBA aa 50-150.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human AJUBA aa 50-150. The exact immunogen used to generate this antibody is proprietary information.Q96IF1

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab241957 was affinity purified using an epitope specific to Ajuba immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ajuba also known as Juba or 8 Ajuba is a LIM domain protein with a molecular mass of around 55 kDa. This protein shows expression in various tissues including the skin and immune cells. Mechanically Ajuba functions as a scaffold protein interacting with several other proteins to regulate cellular processes. It helps in the assembly and stabilization of protein complexes especially at the cell surface and in the nucleus playing an important role in connecting cytoskeletal components to signaling pathways.
Biological function summary
Ajuba influences cell proliferation adhesion and migration. As part of the Ajuba-Zyxin-LIMD1 complex it interacts with actin filaments and diverse cytoskeletal proteins mediating signal transduction from extracellular cues to the nucleus. Through these interactions Ajuba modulates gene expression essential for cell cycle progression and differentiation emphasizing its significance in development and tissue maintenance.
Pathways
Ajuba participates in the Wnt signaling and the Hippo pathway. Within Wnt signaling Ajuba partners with key proteins like β-catenin to regulate transcriptional activity and cell fate determination. Additionally in the Hippo pathway Ajuba associates with large tumor suppressor kinases (LATS) and YAP influencing cellular growth and apoptosis. These pathways demonstrate Ajuba's involvement in maintaining cellular homoeostasis and response to environmental stimuli.
Ajuba's dysregulation associates with cancer and autoimmune diseases. In cancer altered Ajuba expression affects cell survival and proliferation tying it to proteins such as β-catenin in colorectal cancer progression. In autoimmune diseases improper Ajuba functioning disrupts immune cell signaling and tolerance impacting proteins like NF-kB. These connections highlight Ajuba's role in pathological conditions and its potential as a therapeutic target.