Abcam, ab243066, Anti-Malondialdehyde antibody [11E3]

Size: 100µg
Anti-Malondialdehyde antibody [11E3] (ab243066) is a mouse monoclonal antibody detecting Malondialdehyde in Western Blot, IHC-P .
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:11E3,
Isotype:IgG1,
Carrier free:No,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:Specific for MDA conjugated proteins. Does not detect free MDA. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, 4-HHE, 4-HNE, or Methylglyoxal modified proteins.

Product details:
What is this antibody validated in?
Anti-Malondialdehyde antibody [11E3] (ab243066) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P).

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from TCS., Storage buffer-Preservative: 0.09% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Malondialdehyde often abbreviated as MDA is a reactive organic compound formed by the degradation of polyunsaturated lipids. This breakdown occurs through lipid peroxidation which results in MDA being a well-recognized marker for oxidative stress. The molecular mass of malondialdehyde is relatively low around 72.06 g/mol. This small molecule does not localize to specific tissues but instead it can be found throughout the body wherever lipid peroxidation occurs making it widespread in biological systems.
Biological function summary
This compound plays an important role in signaling processes related to oxidative stress. MDA is not part of a protein complex but it interacts with proteins by forming adducts with lysine and histidine residues. Such adduct formations may alter protein function and structure providing insights into oxidative modifications of biomolecules. In research settings scientists often use malondialdehyde tests such as the malondialdehyde ELISA kit to quantify MDA levels as a sign of oxidative damage in cells and tissues.
Pathways
Malondialdehyde integrates into the lipid peroxidation pathway. This pathway functions as a feedback loop indicating oxidative stress conditions within cells. Another related pathway includes the arachidonic acid metabolism. MDA formation signals further lipid peroxidation impacting the interaction with several proteins including cyclooxygenases which are involved in inflammation processes.
Malondialdehyde serves as a biomarker for conditions associated with oxidative stress such as cardiovascular diseases and neurodegenerative disorders. Studies have linked elevated MDA levels with cardiovascular diseases where it can interact with low-density lipoproteins marking them for uptake by macrophages. Similarly in neurodegenerative disorders like Alzheimer's malondialdehyde's levels correlate with protein dysfunctions. These are also often observed in conjunction with beta-amyloid interactions contributing to disease pathology. Researchers frequently employ MDA to KDAs in detailed analyses to understand these processes better.