Abcam, ab243688, Human Relaxin-2 ELISA Kit

Size: 1 x 96Tests
Human Relaxin-2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Relaxin-2 with a sensitivity of 1.05 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 1.05 pg/mL,
Range:2.03 - 130 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Relaxin-2 ELISA Kit (ab243688) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Relaxin-2 protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Relaxin-2 with 1.05 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Relaxin 2 (RLN2) also known as human relaxin is a peptide hormone with a molecular mass of approximately 6 kDa. It belongs to the insulin superfamily. This hormone is secreted primarily by the corpus luteum during pregnancy but also gets expression in other tissues like the heart and kidney. Relaxin 2 plays an important role in reproductive physiology facilitating a range of mechanical processes essential for pregnancy such as remodeling the extracellular matrix and influencing the dilation of the cervix.
Biological function summary
The role of human relaxin involves modulating tissue growth and remodeling. It works by interacting with relaxin family peptide receptors mainly RXFP1. It is not part of a larger complex but has autonomous structural and functional capabilities. The hormone influences collagen metabolism and fibroblast activity enhancing the extensibility and elasticity of the tissues where it is active.
Pathways
Researchers have identified relaxin 2 as a critical component in the relaxin signaling pathway and involved in the transforming growth factor-beta (TGF-beta) pathway. Its interaction with RXFP1 activates several intracellular signaling cascades including adenylate cyclase/cAMP pathway and phosphatidylinositol 3-kinase pathway affecting proteins like collagen and matrix metalloproteinases. These pathways play a fundamental role in connective tissue remodeling and have implications in normal and pathological states.
Studies connect relaxin 2 to conditions such as cardiac fibrosis and scleroderma. In cardiac fibrosis relaxin 2 influences matrix metalloproteinases and collagen metabolism aiming to reduce pathological tissue stiffness. In scleroderma relaxin 2's potential to modulate excessive collagen deposition links it to mitigating disease symptoms alongside proteins like TGF-beta which play a part in the disease progression.