Abcam, ab244279, Anti-S2P antibody

Size: 100µL
Rabbit Polyclonal S2P antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MBTPS2 aa 250-400.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human MBTPS2 aa 250-400. The exact immunogen used to generate this antibody is proprietary information.O43462

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
S2P also known as Site-2 protease or IMP1 is an intramembrane zinc metalloprotease with a mass around 52 kDa. It carries out the proteolytic cleavage of membrane-bound transcription factors. S2P resides in cellular locations like the endoplasmic reticulum membrane and is highly expressed in tissues such as the liver and muscle. This protease acts after the initial cleavage by Site-1 protease (S1P) playing an important role in regulated intramembrane proteolysis.
Biological function summary
S2P cleaves specific substrates involved in lipid metabolism and stress response. This protease is an integral component of the sterol-regulatory element-binding proteins (SREBPs) complex. The cleavage by S2P releases the active form of SREBPs which translocates into the nucleus to regulate genes responsible for cholesterol and fatty acid synthesis. S2P ensures that these transcription factors maintain lipid homeostasis and adapt to cellular environment changes.
Pathways
S2P significantly influences cholesterol biosynthesis and unfolded protein response pathways. In the cholesterol biosynthesis pathway it regulates the activation of SREBPs which in turn controls cholesterol and lipid biosynthesis genes. Additionally in the unfolded protein response pathway it interacts with proteins such as ATF6 to help manage endoplasmic reticulum stress. These interactions highlight S2P's pivotal role in maintaining cellular balance under varying conditions.
S2P dysfunction can lead to conditions like hypercholesterolemia and metabolic syndrome. Impaired S2P activity disrupts normal cholesterol regulation potentially causing elevated cholesterol levels and related cardiovascular diseases. S2P also connects to ATF6 in response to stress and any disturbances might influence metabolic pathways contributing to metabolic syndrome. Understanding the role of S2P in these diseases offers insights into potential therapeutic approaches for managing lipid-related disorders.