Abcam, ab244383, Anti-NCKAP1 antibody

Size: 100µL
Rabbit Polyclonal NCKAP1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NCKAP1 aa 900-1000.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human NCKAP1 aa 900-1000. The exact immunogen used to generate this antibody is proprietary information.Q9Y2A7

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NCKAP1 also known as NAP1 serves an important role mechanically as part of the WAVE regulatory complex. It has a molecular weight of approximately 140 kDa. NCKAP1 is expressed in various tissues with higher levels noted in brain and muscle tissues. It functions in actin cytoskeleton organization by regulating the activation of the Arp2/3 complex which initiates actin polymerization.
Biological function summary
NCKAP1 contributes to cell motility and morphology by participating in lamellipodia and filopodia formation. It forms part of the WAVE complex which includes proteins such as ABI CYFIP and WASP-family proteins. This complex plays a role in rearranging the actin cytoskeleton necessary for various cell movements and shape changes important in processes like cell migration and wound healing.
Pathways
NCKAP1 interacts within the Rac1 signaling pathway. Its actions in the Rac1 pathway are important for membrane ruffling and cell spreading. NCKAP1 is also linked to the mTOR pathway through its role in regulating cytoskeletal dynamics. NCKAP1 activities are associated with other proteins like the p21-activated kinase (PAK) and Filamin A which collaborate in these signaling cascades to execute cellular responses.
NCKAP1 has been implicated in neurological disorders like autism spectrum disorder and schizophrenia. Aberrant actin dynamics regulated by NCKAP1 may contribute to the synaptic dysfunctions observed in these conditions. Furthermore its association with disease-related proteins like CYFIP1 highlights its relevance in these pathologies. Researchers continue to explore how NCKAP1's regulation of the cytoskeleton might influence disease pathways seeking potential therapeutic interventions.