Abcam, ab244502, Anti-IST1 antibody

Size: 100µL
Rabbit Polyclonal IST1 antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human IST1 aa 1-100.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human IST1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.P53990

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
IST1 also known as Increased Sodium Tolerance 1 is a protein involved in membrane trafficking within cells. It weighs around 37 kDa and expresses mainly in the cytoplasm of various cell types especially in tissues with high endocytic and exocytic activity. Researchers often study IST1 to understand its role in the endosomal sorting process. In addition to its presence in human tissues IST1 shows conservation across different species highlighting its importance in cellular functions.
Biological function summary
The IST1 protein operates as a regulator within the ESCRT (endosomal sorting complexes required for transport) machinery. This complex plays a role in the sorting of ubiquitinated cargoes into multivesicular bodies influencing their fate in lysosomal degradation. IST1 interacts with other proteins in the ESCRT-III complex facilitating the proper scission of membrane necks during vesicle budding processes. Its function finds relevance in maintaining cellular homeostasis by orchestrating the turnover of membrane proteins and lipids.
Pathways
IST1 participates in the ESCRT pathway which is integral for endosomal transportation and membrane reformation processes such as cytokinesis and autophagy. In this context IST1 interacts with proteins such as CHMP1A and CHMP1B serving a critical role in the endosomal sorting and degradation pathway. Proper functioning of IST1 is necessary for the correct execution of membrane repair processes aiding in cellular recycling mechanisms.
IST1 exhibits links to neurodegenerative disorders and viral infections. Its role in membrane trafficking connects it to Alzheimer's disease through interactions with proteins like APP and PSEN1 which are related to amyloid-beta plaque formation. Furthermore viruses exploit the ESCRT machinery including IST1 for viral budding and release influencing the progression of viral diseases. Researchers continue to explore IST1's potential as a therapeutic target in such conditions given its involvement in these critical cellular mechanisms.