Abcam, ab244720, Anti-Soluble TNF Receptor I antibody [EPR20675-20] - BSA and Azide free (Capture)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal TNF Receptor I antibody. Carrier free. Suitable for sELISA and reacts with Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20675-20,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Rat,
Applications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab244720 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Rat TNFR1. The recommended pair for sandwich ELISA is:
Capture: ab244720, Rat TNFR1 Capture Antibody (unconjugated)
Detector:
ab244987
, Rat TNFR1 Detector Antibody (unconjugated)
The reference range value is 15.6 - 1000 pg/ml.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Soluble TNF Receptor I also known as sTNFR1 or sTNFR55 functions as a decoy receptor for tumor necrosis factor (TNF). It binds to members of the TNF ligand family particularly TNF-alpha preventing them from interacting with cell-bound receptors. This receptor has a molecular weight of approximately 30 kDa. You will find sTNFR1 expressed in various tissues including lung liver and kidney. sTNFR1 circulates in blood where it plays a role in modulating immune responses.
Biological function summary
Soluble TNF Receptor I is involved in regulating inflammation. It sequesters TNF to control its bioavailability and impede its pro-inflammatory effects. sTNFR1 is part of a larger signaling complex which also includes other TNF receptors and ligands within the TNF receptor superfamily. This ability to regulate TNF activity suggests its important role in maintaining immune system balance and preventing excessive inflammation.
Pathways
Soluble TNF Receptor I modulates key inflammatory pathways including the NF-kB and MAPK pathways. These pathways are critical in the cellular response to stress apoptosis and survival. By binding TNF-alpha sTNFR1 interferes with TNF-alpha's interaction with TNFR1 and TNFR2 cell receptors which are vital in signaling through these pathways. TNFR1 particularly mediates a range of cellular responses including apoptosis and survival by influencing downstream kinases and transcription factors.
Soluble TNF Receptor I has associations with inflammatory and autoimmune conditions. In conditions like rheumatoid arthritis sTNFR1 counteracts the excessive activity of TNF an important mediator of inflammation. It is also connected to cancer where dysregulated TNF signaling may contribute to tumor progression. Through these conditions sTNFR1 directly interacts with TNF-alpha and TNFRs drawing a connection between TNF signaling and disease pathogenesis.