Abcam, ab244801, Anti-CYR61 antibody [EPR21133-104] - BSA and Azide free (Capture)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal CYR61/CCN1 antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR21133-104,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab244801 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human/Mouse CYR61. The recommended pair for sandwich ELISA is:
Capture: ab244801, Human/Mouse CYR61 Capture Antibody (unconjugated)
Detector:
ab245074
, Human/Mouse CYR61 Detector Antibody (unconjugated)
The reference range value is 31.3 - 3000 pg/ml for human and 62.5 - 4000 pg/ml for mouse.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CYR61 also known as CCN1 is a secreted protein involved in cell signaling. It belongs to the CCN family of proteins and weighs approximately 42 kDa. This protein is found in various tissues including the skin lungs and blood vessels. CYR61 is involved in cellular activities such as adhesion migration and proliferation by interacting with cell surface receptors. This active participation in cell signaling makes CYR61 essential to multiple cellular processes.
Biological function summary
CYR61 supports angiogenesis and wound healing by promoting cell survival and adhesion. It functions as a significant component of the extracellular matrix and often participates in complex formations with other CCN proteins. These complexes aid in mediating interactions between cells and their environments. Through these mechanisms CYR61 influences various biological outcomes impacting how tissues grow and repair.
Pathways
CYR61 contributes to both the integrin and TGF-beta signaling pathways. In the integrin pathway it interacts with proteins like integrin alphaVbeta3 to influence cell movement and survival. The TGF-beta pathway involves its regulation of cellular responses to growth factors. By playing important roles in these pathways CYR61 maintains essential cellular functions and intercellular communication impacting diverse physiological processes.
CYR61 has links to cancer and fibrosis. Its overexpression can contribute to tumor progression and metastasis often in connection with matrix metalloproteinases during cancer development. In fibrosis CYR61 collaborates with fibroblast growth factor receptors to promote tissue scarring and stiffness. These connections highlight the importance of CYR61 in pathological conditions offering potential targets for therapeutic intervention.