Abcam, ab245199, Anti-CD16 + CD32 antibody [EPR22345] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal FCGR3 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Recombinant fragment - Mouse samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22345,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab245199 is the carrier-free version of
ab228971
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD16/CD32 also known as FcγRIII and FcγRII are types of Fc gamma receptors. CD16 refers to FcγRIII while CD32 refers to FcγRII. These proteins are involved in binding the Fc region of IgG antibodies. CD16 has a mass of about 50-65 kDa whereas CD32 varies around 40-45 kDa depending on its isoform. These receptors are expressed on the surface of various immune cells like natural killer cells macrophages monocytes and some T cells playing important roles in immune responses.
Biological function summary
CD16/CD32 are critical for mediating antibody-dependent cellular cytotoxicity (ADCC) and phagocytosis. These proteins do not generally form a part of a larger complex but interact directly with immunoglobulins. They enhance the ability of immune cells to clear pathogens and infected cells from the body. These receptors contribute to the activation of signaling pathways that promote the destruction of targets opsonized with antibodies thereby linking adaptive and innate immune responses.
Pathways
These receptors influence the immune signaling cascades such as the NF-kB and MAPK pathways. These pathways are important for inflammatory responses and cellular activation. CD16/CD32 interactions lead to pathways that involve downstream signaling molecules like Syk and PI3K. This allows for immune modulation and assists in communicating between different types of immune responses orchestrating a coordinated attack against pathogens.
CD16/CD32 have been connected to autoimmune diseases and certain cancers. These receptors are implicated in disorders like rheumatoid arthritis where their function can become dysregulated contributing to chronic inflammation. In cancers CD16 is important for mediating immune cell interactions in therapies that use antibodies to target cancerous cells. CD32's expression also links to conditions like systemic lupus erythematosus where altered receptor function impacts disease progression often interacting with proteins involved in immune regulation.