Abcam, ab245646, Anti-RBM5 antibody

Size: 100µg
Rabbit Polyclonal RBM5 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human RBM5 aa 750 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human RBM5 aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P52756

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab245646 was affinity purified using an epitope specific to RBM5 immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RBM5 also known as RNA-binding motif protein 5 or LUCA-15 plays a significant role in pre-mRNA splicing regulation. Its molecular mass is approximately 94 kDa. This protein is expressed in various tissues with noticeable levels in the heart liver and lung. RBM5 influences splice site selection during RNA processing impacting gene expression patterns.
Biological function summary
RBM5 functions as a tumor suppressor influencing cell cycle regulation and apoptosis. It forms part of a spliceosomal complex playing a critical role in the alternative splicing of its target mRNAs. This activity is essential for maintaining normal cellular homeostasis especially in rapidly dividing cells. RBM5 interacts with other splicing factors to modulate the inclusion of specific exons impacting overall cell function.
Pathways
The regulation of alternative splicing by RBM5 integrates into the apoptotic and cell cycle pathways. Its interaction with proteins like Fas and Bcl-2 highlights its role in managing programmed cell death. These connections make RBM5 central in ensuring the balance between cell survival and death an important element in cancer suppression. It also collaborates with other splicing proteins in the spliceosomal pathway to coordinate precise RNA maturation.
Mutations or altered expression of RBM5 link to cancer notably lung and breast cancer. Its tumor-suppressive effects in these conditions highlight its importance in maintaining normal cell behavior. Moreover RBM5 interacts with p53 a well-known tumor suppressor protein indicating its coordinated role in preventing aberrant cell proliferation. The dysregulation of RBM5 and subsequent disruption in RNA processing highlights its significance in cancer-related pathways.