Abcam, ab245713, Mouse PTX3 ELISA Kit

Size: 1 x 96Tests
Mouse PTX3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse PTX3 with a sensitivity of 22.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 22.3 pg/mL,
Range:156.25 - 10000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse PTX3 ELISA Kit (ab245713) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PTX3 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PTX3 with 22.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pentraxin 3 (PTX3) also known as TSG-14 or TNF-Inducible Gene 14 Protein is an important component of the immune system. Mechanically PTX3 functions as part of the body's defense mechanism against pathogens and tissue damage. It has a molecular weight of approximately 42 kDa and is expressed in a range of tissues such as the immune cells endothelium and epithelial cells. Upon inflammatory signals these cells secrete PTX3 which quickly identifies microbial molecules and apoptotic cells.
Biological function summary
The role of PTX3 extends beyond simple pathogen recognition. It is involved in the regulation of inflammation and tissue homeostasis. As part of the pentraxin superfamily PTX3 plays a role in the formation of complexes with other molecules such as complement components. These interactions modulate the complement cascade an essential part of the innate immune response and influence the resolution of inflammation. PTX3 also interacts with extracellular matrix components stabilizing tissue structure during immune response.
Pathways
PTX3 integrates into vital immune signaling networks. It actively participates in the complement pathway where it regulates the activity of C1q and C3 key complement proteins. Additionally PTX3 connects with the Toll-like receptor (TLR) signaling pathway. This interaction helps modulate the body's response to microbial infections by influencing the activity of TLR4 among other related proteins which are fundamental in recognizing pathogen-associated molecular patterns.
PTX3 is linked to various inflammatory conditions. High levels of PTX3 often indicate a response to cardiovascular diseases such as atherosclerosis. In this context its interactions with C1q highlight its role in modulating disease progression. PTX3 also relates to autoimmune diseases like rheumatoid arthritis where its increased expression correlates with disease severity. In rheumatoid arthritis PTX3 can interact with pro-inflammatory cytokines like TNF-alpha exacerbating the inflammatory process.