Abcam, ab246012, Anti-PPP1R14B/PNG antibody

Size: 100µg
Rabbit Polyclonal PPP1R14B/PNG antibody. Suitable for IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PPP1R14B aa 1-100.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PPP1R14B aa 1-100. The exact immunogen used to generate this antibody is proprietary information.Q96C90

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab246012 was affinity purified using an epitope specific to PPP1R14B/PNG immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PPP1R14B also known as protein phosphatase 1 regulatory inhibitor subunit 14B or PNG is an important inhibitor of protein phosphatase 1 (PP1). This inhibitor modulates PP1 activity by binding to it resulting in inhibition of its phosphatase activity. The protein has a molecular mass of approximately 17 kDa. PPP1R14B is widely expressed in human tissues with specific abundance in smooth muscle and the brain indicating its role in functions related to these tissues.
Biological function summary
PPP1R14B plays a role in smooth muscle contraction regulation and neuronal signaling. By acting as a regulatory subunit it takes part in several complexes that modulate phosphatase activity important to muscle contraction and neurotransmitter release processes. The inhibition of PP1 by PPP1R14B modulates the phosphorylation status of its substrates which affects the strength and duration of muscle cell contractions and neuronal response.
Pathways
PPP1R14B integrates into signaling cascades such as the cAMP-dependent signaling pathway and the RhoA/ROCK pathway. It influences the regulation of muscular and neuronal excitability by impacting proteins like myosin light chain (MLC) and synapsins which are downstream targets in these pathways. Interaction with these pathways ensures precise control over muscular contraction and synaptic transmission facilitating proper cellular responses to external stimuli.
PPP1R14B has relevance in conditions like hypertension and neurological disorders which involve dysregulated smooth muscle and neuronal activity. In hypertension its interaction with MLC may affect blood vessel tension. In neurological conditions interactions with synapsins can affect neuronal communication and plasticity. Understanding its role and regulation offers insights into potential therapeutic targets for disease management.