Abcam, ab246796, Anti-ILT-4 antibody [EPR22081] - Low endotoxin, Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ILT-4 antibody. Carrier free. Suitable for Flow Cyt, ICC/IF and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22081,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:Flow Cyt, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab246796 is the carrier-free version of
ab224701
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.
What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Endotoxin level is less than 1 EU/ml as determined by the TAL test, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ILT-4 also known as Immunoglobulin-like transcript 4 is a transmembrane protein with a molecular weight of approximately 45 kDa. The protein is part of the LILR family and is sometimes referred to as LILRB2 protein. ILT-4 is mainly expressed on myeloid lineage cells including monocytes and dendritic cells. The expression of ILT-4 is important for the modulation of the immune response. Biotinylated ILT-4 and biotinylated LILRB2 versions are used frequently in research to study their interactions and functions.
Biological function summary
ILT-4 plays an important role in the regulation of immune tolerance and inhibition. It is not part of a complex but interacts with other immune regulatory proteins. By binding to MHC class I molecules ILT-4 inhibits the activation of immune responses preventing over-activation and autoimmunity. The ILT-4 protein is critical for helping myeloid cells to maintain a balanced immune response often restricting unnecessary inflammation and promoting tolerance.
Pathways
ILT-4 influences immune signaling pathways such as those that control T cell receptor signaling. ILT-4 interactions with proteins like CD85d and other LILRA6 proteins showcase its involvement in negative regulatory signaling pathways. These pathways are essential for maintaining immune homeostasis and limiting immune-mediated damage during infections or tissue injury.
ILT-4 is implicated in cancer and autoimmune diseases such as multiple sclerosis. In cancers ILT-4 modulates the immune environment allowing tumors to evade immune surveillance. In the context of autoimmune disorders aberrant ILT-4 expression or function can lead to insufficient immune regulation contributing to disease progression. CD85d and LILRB2 proteins are also associated with these conditions further influencing ILT-4's role in disease mechanisms.