Abcam, ab246817, Cresyl Violet Stain Solution (1%)

Size: 500mL
Cresyl violet stain solution (1%) (ab246817) is a staining solution for visualizing Nissl substance in neurons on formalin-fixed, paraffin-embedded tissue in immunohistochemistry (IHC). - Stains Nissl granules violet and neuroglia nuclei slightly bluer - All regents supplied in ready-to-use format - Compatible with our Lab essentials imaging workflow offering
Key facts
Form:LiquidSee storage information,
Storage buffer:pH: 3 - 3.4Constituents: 1% 3,7-diamino-12H-benzo[b]phenoxazin-12-ium acetate, 0.2% Acetic acid

Product details:
Cresyl Violet Stain Solution (1%) ab246817 is designed for staining Nissl substance in neurons on formalin fixed, paraffin-embedded tissue. Nissl granules are purple, nuclei of neuroglia and endothelial cells are slightly bluer than Nissl granules.
Nissl Substance: Violet.
Neuroglia Nuclei: Violet to Dark Blue.
Control Tissue:
Cerebral Cortex
Procedure:
Working Solutions: If preferred, reagent may be diluted up to 1:10 with Deionized water just before use.
1. Deparaffinize sections and hydrate to distilled water.
2. Apply Cresyl Violet Acetate solution (or working solution) to tissue for 3-5 minutes
3. Rinse quickly in 1 change of distilled water.
4. Dehydrate quickly in absolute alcohol. (Alcohol may remove stain from tissue over time).
5. Clear in Xylene or Xylene substitute and mount in synthetic resin.
Other products for staining tissue sections
Find more kits and reagents in the
special stains guide
, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the
IHC kits and reagents guide

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C