Abcam, ab246885, Anti-LAD1 antibody

Size: 100µL
Rabbit Polyclonal LAD1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LAD1 aa 350 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human LAD1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.O00515

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LAD1 also known as Ladinin-1 is a 74 kDa protein prominently involved in maintaining the structural integrity of the basement membrane zone in epithelial tissues. LAD1 achieves this function by interacting with other structural proteins and contributing to the stability and adhesion of epithelial cells. This protein is expressed mainly in the skin but can also be detected in other epithelial-rich organs. While playing an important role LAD1 does not act alone; rather it works synergistically with cytoskeletal and extracellular components.
Biological function summary
LAD1 contributes to cell adhesion ensuring strong attachment of epithelial cells to the underlying basement membrane. It forms a part of complex networks within the basement membrane zone working together with proteins like collagen and laminin. This interaction is important for cellular architecture and tissue integrity providing essential support for cells in environments subject to constant mechanical stress.
Pathways
LAD1 participates in the pathways that manage cell adhesion and integrity. It is closely associated with the integrin complex pathway which is vital for linking the extracellular matrix to the cell. LAD1 interacts with integrins and other adhesion molecules such as collagen enhancing cellular communication and structural stability. These interactions support tissue integrity enabling cells to resist shearing forces effectively.
Defects or alterations in LAD1 expression or function are related to skin conditions like epidermolysis bullosa. This disorder involves skin fragility due to improper adhesion between dermal and epidermal layers. LAD1's interactions with connective proteins like collagen XVII become disrupted further exacerbating these conditions. Abnormal LAD1 expression can contribute to the development and severity of such disorders highlighting its role in maintaining healthy skin structure.