Abcam, ab247283, Anti-CEBP Alpha/CEBPA antibody [EP708Y] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal CEBP Alpha/CEBPA antibody. Carrier free. Suitable for ChIC/CUT&RUN-seq, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP708Y,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, Flow Cyt (Intra), ChIC/CUT&RUN-seq, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody recognizes isoform1 and isoform 4.

Product details:
ab247283 is the carrier-free version of
ab40761
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CEBP Alpha also known as CEBPA is a transcription factor belonging to the CCAAT/enhancer-binding protein family. It is composed of a molecular mass approximately between 42 to 45 kDa and is characterized by the leucine zipper motif which contributes to its function in dimerization and DNA binding. CEBP Alpha is expressed in various tissues with notable expression in the liver adipose tissue and hematopoietic cells where it plays a significant role in regulating gene expression related to growth arrest and differentiation.
Biological function summary
CEBP Alpha regulates important cellular processes by acting as a transcriptional regulator. It participates in the control of cellular differentiation proliferation and metabolism. CEBP Alpha forms homo- or heterodimers with other proteins of the CEBP family to exert its functions. It induces expression of genes involved in differentiation of myeloid progenitors into granulocytes and macrophages. The protein also promotes adipogenesis by activating genes responsible for the development and function of adipocytes.
Pathways
CEBP Alpha participates actively in signaling pathways that involve cell cycle control and hematopoiesis. In the hematopoietic pathway CEBP Alpha influences the differentiation of progenitor cells into mature blood cells. Additionally it interacts with other proteins such as PU.1 an important regulator in the myeloid lineage and through the MAPK pathway influences growth arrest and differentiation responses. These interactions highlight its essential role in maintaining the balance between cell proliferation and differentiation.
Alterations in the function or expression of CEBP Alpha have consequential links to acute myeloid leukemia (AML) and obesity. Mutations or disruptions in the CEBP Alpha gene can lead to impaired granulocyte differentiation contributing to the pathogenesis of AML. Additionally CEBP Alpha through its role in adipogenesis is associated with metabolic disorders such as obesity. The protein's interaction with other transcription factors like FLT3 and GATA-2 in the context of these diseases highlights its critical involvement in maintaining normal physiological functions.