Abcam, ab247322, Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Dynamin 1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP801Y,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247322 is the carrier-free version of
ab52611
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Dynamin 1 also known as DNM1 is a GTPase with a molecular mass of approximately 96 kDa. It plays a mechanical role in membrane fission during endocytosis. Dynamin 1 is primarily expressed in neuronal tissues where it participates in the formation and release of synaptic vesicles. Its ability to hydrolyze GTP gives it the mechanical force needed to constrict and sever membranes which is vital for efficient synaptic vesicle recycling in neurons.
Biological function summary
Dynamin 1 helps regulate synaptic transmission by controlling vesicular traffic in neurons. As part of a larger protein complex it interacts with various proteins to mediate endocytosis specifically in presynaptic nerve terminals. These interactions ensure that synaptic vesicles are recycled rapidly allowing neurons to maintain communication across synapses. Disruptions in dynamin 1 function can impair synaptic vesicle recycling affecting neurotransmission efficiency.
Pathways
Dynamin 1 is essential in the clathrin-mediated endocytic pathway. This pathway is a major route for internalizing synaptic vesicles and involves cooperation with other proteins such as clathrin and adaptor proteins. Additionally dynamin 1 participates in the actin cytoskeleton dynamics linking it with actin-associated proteins. These pathways highlight its contributions to maintaining synaptic vesicle endocytosis and neurite outgrowth processes.
Alterations in dynamin 1 function are linked to neurological disorders including epilepsy and Huntington's disease. In epilepsy improper functioning of dynamin 1 disrupts synaptic vesicle recycling leading to imbalances in neurotransmitter release. Connections exist between dynamin 1 and huntingtin where their interactions in vesicular trafficking are important for neuronal stability. Malfunctions in these processes often result in neurodegenerative symptoms emphasizing dynamin 1's involvement in sustaining neural health.