Abcam, ab247429, Anti-Rad17 (phospho S656) antibody [EPR3145] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal RAD17 phospho S656 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR3145,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247429 is the carrier-free version of
ab76012
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rad17 also known as CCYC1 is a protein involved in DNA damage checkpoint control. It has an approximate molecular weight of 75 kDa. Rad17 is expressed in various tissues with higher levels in proliferating cells such as cancerous tissues. It functions as a part of the replication factor C (RFC) complex delivering its essential role during the DNA replication and damage response.
Biological function summary
Rad17 plays an important role in maintaining genome stability by activating cell cycle checkpoints. It belongs to the RFC-like complex which identifies DNA damage and initiates the DNA repair process. The complex binds to chromatin and helps recruit the 9-1-1 complex (Rad9-Rad1-Hus1) to sites of DNA damage. This action is important for the cell to halt the cell cycle and commence repair preventing mutations from being passed on during cell division.
Pathways
Rad17 functions in the DNA damage response pathway and is involved in the ATR-Chk1 pathway. Rad17 recruits ATR (ataxia-telangiectasia and Rad3-related protein) to stalled replication forks facilitating the phosphorylation and activation of Chk1 a checkpoint kinase. This signal allows cells to pause the replication process and repair DNA before continuing the cell cycle. Rad17 is closely related to proteins such as ATR and Chk1 through these pathways as they work together to maintain cellular integrity.
Rad17 has links to cancer and genomic instability syndromes. Mutations or malfunctions in Rad17 can disrupt normal checkpoint control and repair mechanisms leading to uncontrolled cell proliferation and tumor development. In cancer Rad17 interacts with proteins such as BRCA1 in pathways that emphasize DNA repair and checkpoint activation. BRCA1 and Rad17 coordinate to preserve genomic stability and disruptions in these proteins' functions can contribute to the onset of certain breast and ovarian cancers.