Abcam, ab247443, Anti-Endo G antibody [EP1665Y] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Endo G antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP1665Y,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247443 is the carrier-free version of
ab76122
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Endonuclease G (Endo G) is an important mitochondrial nuclease known for its role in DNA and RNA metabolism. Endo G is also referred to as endoglycosidase S and has a molecular mass of approximately 28 kDa. This protein is expressed mainly in the mitochondria but can translocate to the nucleus under certain conditions. It functions by cleaving DNA and RNA which facilitates the processing of nucleic acids in the cell. Endo G exhibits endonuclease activity making it a significant factor in nucleic acid regulation.
Biological function summary
This target plays an essential role in mitochondrial DNA fragmentation during apoptosis. Endo G operates independently of caspases highlighting its role as a non-canonical mediator of apoptosis. It becomes active upon cellular stress usually through the release from the mitochondria where it functions as part of a larger apoptotic complex. This characteristic grants it an important function in programmed cell death mechanisms.
Pathways
Endo G integrates into apoptosis and mitochondrial biogenesis. This protein participates in the intrinsic pathway of apoptosis collaborating with other proteins like apoptosis-inducing factor (AIF) to execute cell death. In the context of mitochondrial biogenesis Endo G involvement suggests potential interaction with proteins that oversee DNA replication and transcription influencing both apoptosis and cellular energy pathways.
Defects or dysregulation in Endo G activity associate with various cancer types and neurodegenerative conditions. In cancer increased Endo G levels correlate with heightened apoptosis emphasizing its potential role in cancer therapy and as a prognostic marker. In neurodegenerative diseases like Parkinson's Endo G may contribute to neuronal cell death interacting with proteins involved in mitochondrial homeostasis. These interactions present insights into therapeutic targets for related conditions.