Abcam, ab247579, Anti-RIAM antibody [EPR2806] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal RIAM antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR2806,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247579 is the carrier-free version of
ab92537
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please
contact us
for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RIAM also known as Rap1-GTP-interacting adaptor molecule is a protein involved in intracellular signaling. RIAM has a molecular weight of approximately 76 kDa. It is expressed in various tissues with a significant presence in hematopoietic cells and endothelial cells. This protein plays a part in connecting cell membrane molecules with actin cytoskeleton therefore promoting cellular adhesion.
Biological function summary
RIAM is essential for the integrin-mediated adhesion processes. It functions as a critical adaptor forming complexes with talin and other signaling proteins which facilitate the activation of integrins. Through these actions RIAM contributes to cell migration immune responses and platelet aggregation. Its interactions influence cellular adhesion and communication pivotal for maintaining tissue structure and response to injuries.
Pathways
RIAM is a vital component in the signaling pathways involving the small GTPase Rap1 which regulates integrins and their effect on cell adhesion. Particularly RIAM serves as a bridge between Rap1 and talin facilitating integrin activation. This process plays an essential role in the focal adhesion pathway where proteins like Kindlin also collaborate to modulate cellular adhesion and movement impacting various physiological processes.
RIAM has connections with immune dysfunction and cardiovascular diseases. For example abnormalities in RIAM-mediated integrin activation can lead to improper immune cell trafficking and related immunodeficiencies. In cardiovascular contexts defective RIAM activity might impair endothelial cell function and contribute to vascular diseases. In these scenarios proteins like talin are closely associated with RIAM often sharing roles in the pathogenesis of these disorders.