Abcam, ab247891, Anti-NOXA2/p67phox antibody [EPR5065] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal NOXA2/p67phox antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5065,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247891 is the carrier-free version of
ab109523
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NOXA2 also known as p67phox is a subunit of the NADPH oxidase complex essential in producing reactive oxygen species (ROS). The protein weighs about 67 kilodaltons and is expressed in phagocytic cells like neutrophils and macrophages. NOXA2/p67phox interacts closely with other subunits of the NADPH oxidase complex playing a critical role in oxidative burst which is essential in the immune response against pathogens.
Biological function summary
NOXA2 contributes to superoxide production by facilitating electron transfer in the NADPH oxidase complex. This complex is integral to the immune system's ability to target and destroy infectious agents. NOXA2/p67phox forms a complex with other components such as gp91phox and p22phox which are required for full activation of the oxidase. The coordinated action of these complexes ensures effective ROS production which is a vital component of the defense mechanism of immune cells.
Pathways
NOXA2 participates in the respiratory burst pathway an important element of innate immunity. This pathway involves the conversion of oxygen to superoxide a process requiring precise interaction with cytochrome b558 and the Rac protein. NOXA2 is also related to signal transduction pathways including those modulated by protein kinase C which further regulates its activation during the immune response. The orchestration of these pathways allows for an effective immune defense mechanism.
NOXA2 is implicated in chronic granulomatous disease (CGD) where mutations in the components of the NADPH oxidase complex impair ROS production. This leads to frequent infections due to deficient pathogen clearance. NOXA2 is also linked to inflammatory disorders due to its role in excessive ROS production. In that context it connects with other proteins such as p47phox which are also involved in both normal and aberrant immune responses.