Abcam, ab247943, Anti-p60 katanin antibody [EPR5071] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal p60 katanin antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5071,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247943 is the carrier-free version of
ab111881
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
P60 katanin sometimes called katanin-p60 or KATNA1 is an important protein involved in microtubule severing. The protein has a molecular mass of approximately 60 kDa. It is expressed in various tissues with a high presence in the nervous system. Mechanically p60 katanin disrupts microtubules by hydrolyzing ATP which results in microtubule fragmentation. This activity requires the proper interaction of its two subunits p60 and p80 to execute its full function.
Biological function summary
P60 katanin plays a significant role in cellular processes such as mitosis where it regulates microtubule dynamics. It is part of a larger complex that includes the regulatory subunit p80 katanin which assists in targeting and anchoring. p60 katanin's activity influences the organization of the microtubule network which is vital for intracellular transport and cell division especially in neurons where it affects axon growth and pruning.
Pathways
P60 katanin is involved in the mitotic and neuronal development pathways. It interacts with proteins like spastin and katanin-like proteins within these pathways. In the mitotic pathway it facilitates chromosome segregation and mitotic spindle organization. During neuronal development its microtubule-severing action contributes to remodeling the cytoskeleton therefore aiding in neuron differentiation and migration.
Abnormal p60 katanin activity links to neurological conditions such as Alzheimer's disease and spastic paraplegia. Mutations or dysregulation in p60 katanin or its pathway associates can lead to impaired microtubule dynamics influencing these diseases. In spastic paraplegia p60 katanin's interaction with spastin is particularly notable as both proteins are involved in microtubule maintenance and defects in either can contribute to the disease pathology.