Abcam, ab247955, Anti-Werner's syndrome helicase WRN antibody [EPR6392] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Werner's syndrome helicase WRN antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR6392,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247955 is the carrier-free version of
ab124673
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Werner's syndrome helicase also known as WRN plays a mechanical role in DNA replication and repair. WRN is a 160 kDa protein containing both helicase and exonuclease activities making it unique among RecQ helicases. It unwinds DNA duplexes and resolves complex DNA structures that can arise during replication and repair processes. WRN is expressed in the nucleus of various cell types including fibroblasts where it maintains genome stability. This helicase is critical in processing DNA intermediates to prevent genomic instability.
Biological function summary
WRN functions as a part of larger protein complexes that participate in DNA damage response and repair. It interacts with several proteins involved in the homologous recombination and base excision repair pathways. WRN's unique enzymatic activities enable it to stabilize and process DNA ends during replication fork collapse and double-strand break repair. By operating in concert with proteins such as RAD51 and replication protein A (RPA) WRN ensures accurate replication and repair preventing mutation accumulation.
Pathways
WRN plays a central role in the maintenance of genomic integrity through its involvement in DNA repair pathways and the aging process. It is integral to the process of homologous recombination where it works closely with the MRE11-RAD50-NBS1 (MRN) complex. Additionally WRN has a function in the DNA damage checkpoint pathway collaborating with proteins like ATM and ATR which help to sense damaged DNA and initiate repair processes. These pathways are important for maintaining DNA integrity and preventing cellular senescence.
WRN is closely linked to Werner syndrome and cancer. Mutations in the WRN gene lead to Werner syndrome a rare disorder characterized by premature aging and cancer predisposition. WRN's interaction with tumor suppressor proteins such as p53 connects it to pathways that can contribute to tumorigenesis when dysregulated. Understanding WRN's function and its role in these diseases provides insight into mechanisms of aging and cancer development offering potential therapeutic targets.