Abcam, ab247960, Anti-SCAI antibody [EPR4128] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal SCAI antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4128,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, Flow Cyt (Intra), ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247960 is the carrier-free version of
ab124688
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SCAI also known as Suppressor of Cancer Cell Invasion is a protein involved in the regulation of cell motility. It has a molecular weight of approximately 96 kDa. SCAI is expressed in various human tissues with a notable presence in the heart and skeletal muscle. This protein plays an important role in modulating the dynamic interactions between the cellular cytoskeleton and regulatory proteins directly affecting the cell's ability to move and invade surrounding tissues.
Biological function summary
SCAI influences cell behavior through its interaction with components of the actin cytoskeleton. It serves as an essential part of the complex that suppresses the invasive behavior of cancer cells. By binding with transcription factors like MRTF and influencing their activity SCAI regulates gene expression linked to cellular movement. This regulation helps maintain the structural integrity of tissues and prevents abnormal cellular invasiveness.
Pathways
Suppression of invasive signaling connects SCAI to vital molecular mechanisms. It significantly interfaces with the SRF (serum response factor) signaling pathway directly affecting processes involving actin dynamics and transcriptional regulation. SCAI also interacts with proteins such as MRTF and G-actin playing an important role in these pathways by inhibiting excessive cellular movement maintaining normal cell function.
Links between SCAI and aggressive cancer types such as breast cancer are well-established. Changes in SCAI expression are associated with increased cancer cell invasiveness and metastasis. The protein influences disease processes partly through its interaction with MRTF a critical player in oncogenic pathways. By modulating MRTF's activity SCAI potentially affects cancer progression highlighting its importance in disease regulation.