Abcam, ab247988, Anti-Phospholipase C beta 3/PLCB3 antibody [EPR5951] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Phospholipase C beta 3/PLCB3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5951,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab247988 is the carrier-free version of
ab124735
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Phospholipase C beta 3 often called PLCB3 plays an important role in cellular signal transduction. This enzyme exists as part of the phospholipase C family enhances the conversion of phosphatidylinositol 45-bisphosphate (PIP2) into diacylglycerol (DAG) and inositol triphosphate (IP3). These molecules act as secondary messengers and are important to cellular responses. PLCB3 has a molecular mass of approximately 152 kDa. It exhibits significant expression in various tissues including the brain and heart among others where it regulates numerous physiological processes.
Biological function summary
The activation of PLCB3 influences intracellular calcium levels by producing IP3 contributing to calcium signaling pathways. As part of its biological role PLCB3 contributes to a dynamic signaling complex interacting with receptors like G-protein-coupled receptors (GPCRs) and affecting downstream signaling. This enzyme actively participates in regulating cellular processes such as proliferation and differentiation by mediating signal transduction pathways.
Pathways
PLCB3 integrates into the calcium signaling and phosphatidylinositol signaling pathways where it functions through interaction with G alpha q proteins. The activation of PLCB3 leads to the release of calcium ions from the endoplasmic reticulum a process important for many cellular functions. In these pathways PLCB3 shares interactions with other related proteins like protein kinase C (PKC) which further modulates cellular responses.
Dysregulation of PLCB3 activity has links to several pathophysiological conditions including cardiovascular diseases and cancer. Abnormal PLCB3 signaling correlates with irregular calcium homeostasis impacting cardiac functions and promoting oncogenic transformations. Interactions between PLCB3 and proteins such as GPCRs highlight their importance in developing therapeutic targets for treating these diseases.