Abcam, ab248272, Anti-RAC3 antibody [EPR6680] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal RAC3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Rat, Mouse, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR6680,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248272 is the carrier-free version of
ab129062
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein RAC3 also known as p21-activated kinase 3 or PAK3 is a member of the Rho family of small GTPases. RAC3 has an approximate mass of 22 kDa. This protein functions as a molecular switch that cycles between an active GTP-bound state and an inactive GDP-bound state regulating a variety of cellular processes. RAC3 is expressed in many tissues with prominent expression in the brain muscle and heart. It plays a significant role in controlling the actin cytoskeleton influencing cell shape motility and growth.
Biological function summary
RAC3 influences cell signaling pathways by interacting with other proteins and forming complexes. It participates in the regulation of a variety of physiological processes including cell polarity migration and growth. RAC3 interacts with proteins such as PAK which is important for the phosphorylation of substrates involved in actin reorganization. This activity allows RAC3 to coordinate with other cellular components affecting processes like the migration of neurons during development and immune cell responses.
Pathways
RAC3 is involved in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways play important roles in cell proliferation survival and differentiation. Through the MAPK/ERK pathway RAC3 interacts with proteins like Ras and Raf facilitating the signaling cascade that leads to gene expression changes. In the PI3K/AKT pathway RAC3 contributes to cellular survival and metabolism by interacting with PI3K and AKT promoting pathway signaling important for maintaining cell health under stress.
RAC3 has been linked to cancer and neurodevelopmental disorders. Its role in actin cytoskeleton dynamics and cell signaling makes it relevant in the progression of cancers where its overexpression can enhance metastatic capabilities. RAC3 has connections with the epidermal growth factor receptor (EGFR) which enhances proliferative signals in tumor cells. In neurodevelopmental disorders alterations in RAC3 function can impact neuronal migration and brain development. This connection involves RAC3's interplay with proteins such as PAK3 influencing synaptic development and cognitive functions.