Abcam, ab248286, Anti-LysRS antibody [EPR7921] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal LysRS antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR7921,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248286 is the carrier-free version of
ab129080
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Lysine-tRNA ligase also known as LysRS plays a mechanical role in the translation process by facilitating the attachment of lysine to its corresponding tRNA a critical step for protein synthesis. LysRS is a class II aminoacyl-tRNA synthetase that contributes to the accuracy of genetic code translation. It is expressed in almost all cells as it is essential for protein production. The molecular weight of LysRS is approximately 68 kDa which reflects the complexity of its function and structure.
Biological function summary
Lysyl-tRNA synthetase participates in more than just translation. It is also involved in signal transduction pathways due to its ability to interact with other proteins. LysRS forms a multi-synthetase complex which includes several other aminoacyl-tRNA synthetases and auxiliary proteins. Through this complex it influences functions like gene expression and immune responses. The activity of LysRS outside of its synthetase function shows its versatility within cellular processes.
Pathways
Lysyl-tRNA synthetase is involved in critical pathways like mTOR signaling and aminoacyl-tRNA biosynthesis. These pathways are central to controlling cell growth and survival. LysRS works closely with mTOR a central protein that senses nutrient availability and cellular energy influencing the synthesis of proteins in response to cellular conditions. The proper functioning of these pathways is essential for cellular adaptation and responsiveness.
Lysyl-tRNA synthetase plays a significant role. Diseases like Charcot-Marie-Tooth disease and cancer are associated with LysRS mutations and dysregulation. In particular Charcot-Marie-Tooth disease showcases the involvement of LysRS through dysfunctional protein synthesis affecting peripheral nerves. LysRS's connection to cancer involves its interaction with proteins like mTOR which can influence cancer progression through altered cell growth pathways. Understanding LysRS's role provides insights into the mechanisms behind these diseases.