Abcam, ab248297, Anti-Glucosidase 2 subunit beta antibody [EPR8047] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Glucosidase 2 subunit beta antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR8047,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248297 is the carrier-free version of
ab129098
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glucosidase 2 subunit beta also known as PRKCSH plays an important role in protein processing within the endoplasmic reticulum (ER). This protein forms part of the heterodimeric enzyme complex glucosidase II responsible for cleaving glucose molecules during glycoprotein maturation. With a molecular weight of about 60 kDa glucosidase 2 subunit beta is important for efficient N-linked glycosylation which is a fundamental process for protein folding and quality control. This protein localizes mainly to the ER where it contributes to proper cellular functions by aiding in the preparation of proteins for trafficking to their final destinations.
Biological function summary
Glucosidase 2 subunit beta is associated with protein maturation and quality control within the ER lumen. It is part of the glucosidase II complex which functions to trim glucose residues from N-linked oligosaccharides on nascent glycoproteins facilitating their proper folding and preventing misfolded proteins' accumulation. This activity ensures that only properly folded proteins progress through the secretory pathway while faulty proteins are targeted for degradation. Through these processes glucosidase 2 subunit beta supports cellular homeostasis and protein turnover.
Pathways
Glucosidase 2 subunit beta plays an influential role in the protein processing and ER-associated degradation (ERAD) pathways. The protein operates within these pathways to regulate glycoprotein folding impacting the unfolding protein response (UPR). It interacts with several proteins including calnexin and calreticulin which are key players in the glycoprotein folding process. Moreover the proper function of glucosidase 2 subunit beta in these pathways is necessary for maintaining the cellular stress response ultimately ensuring efficient cell function and survival.
Defects in glucosidase 2 subunit beta have links to autosomal dominant polycystic liver disease (PCLD). Mutations in PRKCSH which encodes glucosidase 2 subunit beta can lead to the development of PCLD due to disrupted glycoprotein folding and ER stress-induced apoptosis. Additionally abnormalities in its function may indirectly connect to diabetes mellitus by affecting insulin receptor glycosylation and secretion processes. These relationships illustrate the broader impact of glucosidase 2 subunit beta on health and disease providing insight into potential therapeutic targets for treatment strategies.