Abcam, ab248576, Anti-N4BP1 antibody [EPNCIR118] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal N4BP1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPNCIR118,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat,
Applications:Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248576 is the carrier-free version of
ab133610
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Michael Kuehn.
View antibodies from NCI Center for Cancer Research Collaboration
Species reactivity
Human: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
N4BP1 also known as NEDD4-binding protein 1 is a protein with a molecular mass of approximately 67 kDa. It is ubiquitously expressed with notable levels in kidney liver and brain tissues. Mechanically N4BP1 acts as an adaptor molecule involved in protein-protein interactions especially noted for its role in ubiquitination processes. The protein has interactions with the E3 ubiquitin ligase NEDD4 influencing protein turnover and cellular homeostasis.
Biological function summary
N4BP1 plays a role in cellular signaling pathways that maintain protein stability and degradation. It is part of multiprotein complexes mediating ubiquitin-dependent protein processing. Through these complexes N4BP1 participates in the regulation of protein levels impacting various cellular processes such as cell cycle progression and stress response.
Pathways
N4BP1 is integral to the ubiquitin-proteasome system a critical pathway for protein catabolism in cells. It interacts with components of the NEDD4-mediated pathway impacting proteins such as SMAD4. This modulation affects signal transduction processes that control cellular fate decisions. Additionally N4BP1 is involved in pathways regulating inflammatory responses linking it to immune response modulation.
N4BP1 has associations with cancer and neurodegenerative diseases. Alterations in N4BP1's function or expression can affect the ubiquitination process influencing tumor suppressor proteins and cellular proliferation in cancer. In neurodegenerative conditions N4BP1's interactions with ubiquitin-related proteins such as NEDD4 play a role in protein aggregation dynamics contributing to disease pathology by potentially impacting the clearance of misfolded proteins.