Abcam, ab248682, Anti-LSP1 antibody [EPR3160] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal LSP1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR3160,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248682 is the carrier-free version of
ab134099
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Lymphocyte-specific protein 1 also known as LSP1 is a 52 kDa intracellular F-actin binding protein. It plays a role in cytoskeletal organization and cell motility. This protein has significant expression in lymphocytes neutrophils macrophages and endothelial cells. LSP1 modulates several cellular functions including adhesion and migration by interacting with the actin cytoskeleton. Known alternatively as pp52 and WD-40 repeat-containing protein 1 LSP1 acts as a link connecting signal transduction from cell surface receptors to cytoskeletal regions.
Biological function summary
LSP1 influences immune cell regulation and inflammatory response. By acting as an important player in the modulation of actin polymerization it contributes to leukocyte chemotaxis and transmigration. LSP1 forms part of a larger protein complex involved in reorganizing the cytoskeleton facilitating cellular dynamics necessary for immune response. Additionally interactions with adapter proteins like 14-3-3 add another level of functional regulation.
Pathways
LSP1 integrates into the chemokine signaling pathway and the P38 MAPK signaling pathway. In these pathways it exerts influence on cellular processes such as migration and response to external stimuli. It shows connections with proteins such as CXCR4 and MAPK highlighting its role in conveying chemotactic signals that direct immune cells toward inflammatory sites. These interactions are integral in establishing directionality and speed of cell movement in response to chemokine gradients.
Aberrations in LSP1 function or expression link to autoimmune conditions like systemic lupus erythematosus and vascular disorders like atherosclerosis. Its role in mediating vascular permeability and leukocyte migration suggests that dysregulation can contribute to pathogenic inflammation. Related proteins like MCP-1 and ICAM-1 participate in these disease pathways highlighting the involvement of LSP1 in modulating endothelial responses and immune cell recruitment at sites of inflammation.