Abcam, ab248899, Anti-HIP1R antibody [EPR9437] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal HIP1R antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR9437,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab248899 is the carrier-free version of
ab140608
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HIP1R also known as Huntingtin-interacting protein 1 related is an endocytic adaptor protein. This protein weighs approximately 116 kDa. It primarily facilitates clathrin-mediated endocytosis by linking endocytic vesicles to the actin cytoskeleton. HIP1R is enriched in neuronal tissues particularly within the brain and is present in other tissues like muscle and liver. The protein contains a domain structure having an ENTH domain and coiled-coil regions which allow it to interact with other cellular components.
Biological function summary
HIP1R contributes to membrane trafficking and actin dynamics. As part of the endocytic machinery HIP1R directly interacts with clathrin and actin fostering vesicle motility and shaping membrane curvature. The protein resides within a larger protein complex that includes clathrin actin and other endocytic proteins. Its role in vesicle formation and transport is essential for maintaining cellular homeostasis by controlling receptor internalization and sorting.
Pathways
HIP1R participates in the clathrin-mediated endocytosis and actin cytoskeleton regulation pathways. It shares functional connections with proteins such as clathrin and actin emphasizing its role in vesicular dynamics and membrane trafficking. The protein ensures effective communication and transport within cells by coordinating with these pathways. These processes contribute to essential cellular functions like nutrient uptake and signal transduction.
HIP1R associates with neurodegenerative conditions and cancer. Altered expression of HIP1R can occur in pathological states impacting endocytic and cytoskeletal functions. Its interaction with Huntingtin another significant protein connects it to Huntington’s disease where proper endocytic action becomes disrupted. In cancer dysregulation of endocytosis-related proteins including HIP1R leads to aberrant cell signaling and proliferation linking HIP1R to oncogenic pathways.