Abcam, ab249122, Anti-PPP2R1B antibody [EPR10158] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal PPP2R1B antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR10158,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249122 is the carrier-free version of
ab154815
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein PPP2R1B also known as Protein Phosphatase 2 Scaffold Subunit A beta isoform acts as a scaffold for the assembly of PP2A complexes. PPP2R1B has a molecular mass of approximately 66 kDa. The protein expresses widely across various tissues with notable presence in the brain heart and skeletal muscle. As a scaffold subunit PPP2R1B coordinates the structural integrity necessary for phosphatase activity by recruiting regulatory and catalytic subunits important to its function.
Biological function summary
PPP2R1B integrates into the PP2A holoenzyme complex a major serine/threonine phosphatase. This complex regulates numerous cellular processes including cell growth division and signal transduction. PPP2R1B plays a role in maintaining cellular homeostasis through its regulatory activities. By doing so it links to various signaling events some of which control oncogenes and tumor suppressors demonstrating the protein's influence over cell cycle regulation and apoptosis.
Pathways
PPP2R1B plays a significant role in the Raf/MEK/ERK signaling pathway and the PI3K/AKT pathway. Through these pathways PPP2R1B interacts with PDPK1 and AKT1 modulating their phosphorylation status and thereby affecting cell proliferation and survival. By influencing such pathways PPP2R1B helps determine cellular responses to external stimuli impacting processes like differentiation and metabolism that are vital for organismal function.
PPP2R1B links to cancer and Alzheimer's disease. Mutations or downregulation in PPP2R1B have been implicated in colorectal cancer where it alters the PP2A activity and allows unregulated cellular proliferation. In Alzheimer's disease PPP2R1B's interaction with tau protein goes awry contributing to neurofibrillary tangle formation. These connections highlight the importance of PPP2R1B in understanding the molecular basis of these diseases and potential therapeutic targets.