Abcam, ab249240, Anti-GALE antibody [EPR11088(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal GALE antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11088(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249240 is the carrier-free version of
ab155997
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
'GALE' also known as UDP-galactose-4-epimerase is an enzyme that plays a critical role in carbohydrate metabolism. It weighs approximately 38 kDa and is expressed in various tissues across the body. This enzyme is responsible for the interconversion of UDP-galactose and UDP-glucose essential in the Leloir pathway of galactose metabolism. GALE's activity is necessary for maintaining balanced sugar levels and genetic mutations in this protein can lead to significant metabolic issues.
Biological function summary
UDP-galactose-4-epimerase facilitates the conversion of galactose-1-phosphate to UDP-galactose a reaction occurring in the utilization of galactose obtained from diet. This enzyme is not part of a larger protein complex but operates independently to mediate the transformation of sugars. Its activity helps in the synthesis of glycoproteins and glycolipids which are fundamental to various cellular functions including cell signaling and adhesion.
Pathways
GALE is an important component in the galactose metabolism pathway. It ensures the proper function of carbohydrate interconversion processes important for energy production and biomolecule synthesis. GALE interrelates with other enzymes like GALT (galactose-1-phosphate uridyltransferase) and GALK1 (galactokinase 1) which together facilitate the orderly breakdown and use of galactose in cells. Disruption in its function can affect these interconnected pathways leading to metabolic imbalances.
Deficiencies or mutations in GALE lead to type III galactosemia which is characterized by the body's inability to properly manage galactose. This genetic disorder can cause symptoms such as jaundice cataracts and developmental delays. GALE's relationship with GALT another enzyme implicated in classic galactosemia (type I) highlights an interconnected network where disruptions in similar pathways can result in metabolic dysfunctions. Careful monitoring of these enzymes is necessary in managing and understanding the metabolic disorders tied to galactose metabolism.