Abcam, ab249243, Anti-Rapsyn antibody [EPR9759] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Rapsyn antibody. Carrier free. Suitable for WB and reacts with Rat, Mouse samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR9759,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249243 is the carrier-free version of
ab156002
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rapsyn also known as receptor-associated protein of the synapse or 43 kDa postsynaptic protein has a mass of 43 kilodaltons. Rapsyn is mainly expressed in skeletal muscle tissues. Mechanically it plays a role as a scaffolding protein that links nicotinic acetylcholine receptors (nAChRs) to the postsynaptic cytoskeleton at neuromuscular junctions. This association ensures proper clustering of nAChRs which is essential for efficient synaptic transmission.
Biological function summary
Rapsyn facilitates the organization and stabilization of acetylcholine receptors at the neuromuscular junction. It acts as part of a larger protein complex that anchors these receptors to the skeletal muscle surface. Macromolecular complexes like those involving Rapsyn are important for the formation and maintenance of the postsynaptic membrane which ensures effective muscle contraction in response to nerve signals. Additionally Rapsyn assists in the aggregation of receptors during synaptogenesis the process by which synapses form between neurons and muscle fibers.
Pathways
Rapsyn contributes to signal transduction and synaptic transmission processes. It associates closely with Dystrophin-glycoprotein complex (DGC) pathway maintaining the structural integrity of the neuromuscular junction. Rapsyn interacts with proteins such as MuSK (Muscle-Specific Kinase) to facilitate nAChR clustering during synapse formation. This interaction also involves agrin-mediated signaling which is critical in neuromuscular development and function.
Mutations or dysfunctions in Rapsyn can be linked to conditions such as congenital myasthenic syndromes (CMS). CMS involves impaired neuromuscular transmission causing muscle weakness and fatigue. Rapsyn mutations can disrupt its interaction with proteins like nAChRs and MuSK compromising synaptic stability and efficiency. Another related disorder is limb-girdle muscular dystrophy where pathways involving Rapsyn and DGC proteins are affected leading to progressive muscle weakness and degeneration.