Abcam, ab249255, Anti-CYFIP1 antibody [EPR10782] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal CYFIP1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR10782,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249255 is the carrier-free version of
ab156016
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CYFIP1 also known as cytoplasmic FMR1-interacting protein 1 plays an important role in cellular processes including actin dynamics and translation regulation. This protein which has a mass of approximately 140 kDa interacts with FMRP and links to the ribosome controlling mRNA translation. CYFIP1 is expressed mostly in the brain but is also present in other tissues like blood. Researchers study CYFIP1 to understand its role in neural activity and cellular signaling processes.
Biological function summary
CYFIP1 operates as part of the WAVE regulatory complex which is critical for actin polymerization. This complex facilitates cytoskeletal reorganization essential for cellular movement and morphology. CYFIP1 influences synaptic plasticity an important process in neural signaling and cognitive functions. By interacting with other molecular components CYFIP1 impacts processes like memory formation and learning.
Pathways
CYFIP1 is integral to the Rac1 and Arp2/3 pathways which directly affect actin filament assembly. In these pathways CYFIP1 interacts with proteins such as NCKAP1 and ABI2 which play roles in the regulation of actin cytoskeleton reorganization. The involvement of CYFIP1 in these pathways highlights its importance in processes that require rapid cytoskeletal changes such as cell migration and synaptic growth.
CYFIP1's function draws a connection to neurodevelopmental disorders and schizophrenia. Alterations in the expression of CYFIP1 have been observed in these conditions suggesting a link between its dysregulation and abnormal brain development. Moreover its interaction with the FMRP protein ties it to Fragile X syndrome characterized by cognitive impairment. Research into these associations provides valuable insight into potential therapeutic targets for these complex conditions.