Abcam, ab249313, Anti-PIGS antibody [EPR11275(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal PIGS antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11275(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249313 is the carrier-free version of
ab157211
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PIGS also known as Phosphatidylinositol Glycan Anchor Biosynthesis Class S is a protein involved in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors. It is a component of the GPI transamidase complex which plays an important role in attaching GPI anchors to proteins. PIGS has a molecular mass of approximately 99 kDa and is predominantly expressed in the endoplasmic reticulum. This protein ensures the proper anchoring of GPI-anchored proteins to the membrane which is essential for their function.
Biological function summary
The GPI anchor system is critical for membrane protein localization and function. PIGS is an integral part of a multimeric enzyme complex within the endoplasmic reticulum that facilitates the attachment of GPI anchors to proteins. This process affects various cellular mechanisms including signal transduction and cell adhesion. Proper function of PIGS and its associated complex is necessary for maintaining the stability and activity of GPI-anchored proteins.
Pathways
The role of PIGS is important in the GPI-anchor biosynthesis pathway. This pathway involves a series of reactions that result in the addition of a GPI anchor to proteins facilitating their sorting and targeting to specific cellular locations. PIGS is part of this pathway along with other proteins like GPAA1 and PIGT which work together in the GPI transamidase complex to attach newly synthesized GPI anchors to proteins.
Malfunction of PIGS or disruptions in GPI anchor biosynthesis can lead to health issues such as paroxysmal nocturnal hemoglobinuria (PNH) and hyperphosphatasia with mental retardation syndrome (HPMRS). In PNH defects in GPI-anchor synthesis lead to increased sensitivity of red blood cells to complement-mediated lysis. PIGS interacts with proteins such as CD59 and DAF which rely on GPI anchors to inhibit complement pathway activation and ensure cell protection.