Abcam, ab249422, Anti-H Cadherin antibody [EPR9621] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal H Cadherin antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR9621,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249422 is the carrier-free version of
ab167407
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
H Cadherin also known as Cadherin-13 or T-cadherin is a unique member of the cadherin family of cell adhesion molecules. It weighs approximately 95 kDa. Unlike classical cadherins H Cadherin lacks the transmembrane and cytoplasmic domains anchoring instead to the membrane via a glycosylphosphatidylinositol (GPI) anchor. H Cadherin expresses in various tissues including endothelial cells cardiac muscle and neurons suggesting its versatile role in different biological contexts.
Biological function summary
The protein plays a role in cell-cell interactions and tissue organization without forming typical adherens junctions. Researchers have identified H Cadherin as a part of the significant complex involved in neural network formation vascular patterning and cardiac tissue integrity. In neurons its expression modulates axon growth and guidance. In the cardiovascular system it contributes to heart function and blood vessel maintenance through its adhesive properties and involvement in signaling pathways.
Pathways
Several biological networks incorporate H Cadherin as an important element. Notably it participates in the Wnt signaling and PI3K/AKT pathways. H Cadherin interacts with proteins such as β-catenin in the Wnt pathway influencing cellular responses like proliferation and differentiation. In the PI3K/AKT pathway the protein may affect cell survival and metabolism. These interactions illustrate H Cadherin's involvement in important cellular processes.
H Cadherin has significant associations with cardiovascular diseases and certain cancers. Altered expression levels or mutations in H Cadherin can disrupt cellular adhesion and signaling contributing to the progression of atherosclerosis and tumor formation. In cancer linkages between H Cadherin and other cadherin family members like E-cadherin suggest complex interactions that can modulate tumor cell behavior influencing invasion and metastasis.