Abcam, ab249576, Anti-NMD3 antibody [EPR11979(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal NMD3 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11979(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249576 is the carrier-free version of
ab170898
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NMD3 also known as Ribosome Export Adaptor NMD3 serves as an important shuttling protein involved in the nuclear export of the 60S ribosomal subunit. It has an estimated mass of approximately 60 kDa. The expression of NMD3 is widespread but prominently seen in cells with high proliferative activity. This protein resides in the nucleoplasm and nucleolus but translocates to the cytoplasm to facilitate its function.
Biological function summary
NMD3 plays a role in ribosomal subunit synthesis by acting as an adaptor required for the export of the mature 60S subunit from the nucleus to the cytoplasm. It does not act alone; NMD3 collaborates with several other proteins forming a transient complex with certain nucleoporins and exportins. This dynamic engagement ensures the correct timing and location of ribosome assembly a vital process for protein synthesis.
Pathways
NMD3 integrates into the ribosome biogenesis pathway an essential process for cellular growth and division. It interacts with larger complexes like the nuclear pore complex and partners with various proteins such as the exportin Crm1. NMD3’s role ensures the appropriate transport and assembly of the ribosomal subunits impacting overall protein translation efficiency.
NMD3 has connections to ribosomopathies and certain cancers. Dysregulation of NMD3 function can lead to impaired ribosomal assembly contributing to defective protein synthesis. The disruption in its function often links to overexpression or mutations in associated proteins like the nucleoporins which can contribute to the pathogenesis observed in specific cancers. Understanding the modifications in NMD3 and its associated protein interactions can provide insights into these disease processes paving the way for therapeutic targets.