Abcam, ab249597, Anti-Calpain 15/SOLH antibody [EPR11078] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Calpain 15/SOLH antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11078,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249597 is the carrier-free version of
ab170926
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Calpain 15 also known as SOLH (small optic lobes homolog) is a member of the calpain family of proteins which are calcium-dependent cysteine proteases. Calpain 15/SOLH has a molecular mass approximately 75 kDa. Researchers have found its expression in various tissues including the brain heart and skeletal muscle. As with other calpain proteins its function hinges on calcium concentration where it undergoes conformational changes to become active and facilitate the cleavage of its substrates.
Biological function summary
Calpain 15 functions as a calcium-regulated enzyme that participates in the modulation of cytoskeletal dynamics. This protein plays roles in cellular processes like migration signal transduction and apoptosis. It does not specifically associate with well-defined complexes but interacts with multiple cellular structures due to its protease activity. By controlling the degradation of specific proteins Calpain 15 regulates the restructuring of the actin cytoskeleton influencing cell shape and movement.
Pathways
Calpain 15 influences both the integrin signaling and apoptosis pathways. In integrin signaling it modifies the cytoskeleton structure for cell movement and stability. It also plays a role in apoptosis by regulating proteins such as Bcl-2 and caspases which are important for cell survival or death decisions. These interactions indicate its part in complex pathway networks that adjust cellular responses to numerous external stimuli.
Researchers have linked Calpain 15 to neurological disorders including Alzheimer's disease by its involvement in neuronal cell death pathways. Its interaction with proteins like caspase-3 can amplify apoptotic signals contributing to neurodegeneration. Additionally aberrant Calpain 15 activity is connected to muscular dystrophy. The proteolytic imbalance caused by altered Calpain 15 levels impacts muscle integrity and function relating it to proteins like dystrophin which is essential for muscle stability.