Abcam, ab249617, Anti-ASAH2B antibody [EPR10539] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ASAH2B antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR10539,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra), IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249617 is the carrier-free version of
ab170949
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ASAH2B also known as neutral ceramidase B is an enzyme involved in the metabolism of sphingolipids. It has a molecular mass of roughly 100 kDa. The enzyme functions by hydrolyzing ceramides into sphingosine and free fatty acid in a neutral pH environment. ASAH2B is expressed in various tissues including the brain kidney and liver. Its activity contributes significantly to maintaining the balance of sphingolipid-mediated cellular processes.
Biological function summary
ASAH2B impacts cell signaling and microsomal integrity by regulating sphingosine levels. It is not known to be part of a larger complex but it still plays an important role in the ceramide catabolic process. Sphingosine produced by ASAH2B can influence many cellular functions such as apoptosis proliferation and differentiation. It modulates the levels of bioactive lipids impacting cellular homeostasis.
Pathways
The enzyme is integral to the sphingolipid metabolism pathway. By breaking down ceramides ASAH2B facilitates interactions in the sphingolipid signaling pathway which is important for cell fate decisions. ASAH2B works in conjunction with proteins like sphingosine kinase which further converts sphingosine into sphingosine-1-phosphate another signaling molecule essential for cell growth and survival.
ASAH2B dysregulation associates with conditions such as neurodegenerative diseases and cancer. Improper ceramide metabolism influenced by ASAH2B activity may contribute to these pathological states. For instance altered levels of ASAH2B can disrupt normal apoptotic signaling pathways linking it to cancer progression. It also interacts with proteins like ceramide synthase which play roles in these diseases by affecting the balance of pro-apoptotic and pro-growth signals.