Abcam, ab249650, Anti-ARF4 antibody [EPR12133(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ARF4 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR12133(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IP, ICC/IF, Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:May cross react with family members, immunogen has 86% homology with ARF5.

Product details:
ab249650 is the carrier-free version of
ab171746
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ADP-ribosylation factor 4 (ARF4) is a member of the ARF family of GTP-binding proteins also known as small GTPases. It has an approximate molecular mass of 21 kDa. ARF4 is expressed in various tissues including the liver kidney and pancreas. Mechanically ARF4 cycles between an active GTP-bound state and an inactive GDP-bound state. This cycling is important for regulating vesicle trafficking and membrane dynamics within cells.
Biological function summary
This protein plays a significant role in vesicular transport processes particularly in the Golgi apparatus. ARF4 forms part of protein complexes involved in trafficking between different organelles. It interacts with coat protein complex I (COPI) and clathrin-coated vesicles which help in cargo selection and budding process of vesicles. These interactions facilitate the movement of proteins and lipids within cells.
Pathways
The protein ARF4 integrates into the vesicular transport and Golgi transport pathways. It interacts with coatomer proteins which are responsible for retrograde transport from Golgi to ER maintaining normal cellular function. ARF4 also shows connection with proteins like ARF1 and ARF5 through these pathways. This involvement underlines its role in normal cellular homeostasis and protein sorting.
Improper functioning of ARF4 links to neurodegenerative diseases and some forms of cancer. Alterations in ARF4 disrupt essential cellular trafficking contributing to diseases like Alzheimer's disease. In cancer abnormal ARF4 activity links with proteins like ARF1 where dysregulation affects cell growth and migration. Understanding these associations is critical to developing targeted therapies.