Abcam, ab249695, Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Calmegin antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human, Common marmoset, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11832-11,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human, Common marmoset,
Applications:Flow Cyt (Intra), IHC-P, ICC/IF, WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249695 is the carrier-free version of
ab172477
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Calmegin also known as CLGN is a protein involved in chaperone activity during spermatogenesis. It has a mass of approximately 65 kDa and is predominantly expressed in the testis. More specifically Calmegin is found in germ cells where it plays a role in the development and maturation of sperm. The protein ensures proper protein folding and stabilization of other proteins during sperm development.
Biological function summary
Calmegin acts as part of a complex necessary for sperm fertility and function. This protein is critical in processing and assembling the molecules required for spermatogenesis. By interacting with other chaperones Calmegin helps to regulate the endoplasmic reticulum's function in germ cells ensuring that proteins reach their target destinations correctly and are folded properly. When functioning as intended these processes facilitate normal sperm development.
Pathways
Calmegin operates within pathways critical to reproductive biology. It is closely tied to the unfolded protein response pathway which maintains protein homeostasis in cells. Additionally it works in concert with proteins like Calnexin in processes that manage protein maturation in the endoplasmic reticulum. All these interactions highlight Calmegin's role in effective protein management during germ cell development affecting male fertility.
Improper function of Calmegin can link to male infertility. Deficiencies or mutations in Calmegin can interfere with spermatogenesis leading to decreased sperm quality or quantity. The protein is also associated with Calreticulin in regulatory functions and abnormalities in these proteins can further impact reproductive health negatively making them critical targets for studies related to infertility treatments.